In addition, no KIT mutation at codon 816 is found in MML, and neoplastic mast cells usually are CD25-negative cells.46 MML can also be discriminated from basophilic leukemia by immuno-phenotyping, as mast cells are KIT+/CD34-/2D7- cells, whereas basophils are KIT-/CD34-/2D7+ cells. This evidence concerns the gene CD34 and acute basophilic leukemia.