The results obtained with S. Enteritidis were similar to those observed in S. Typhimurium - macrophages responded by a significantly higher transcription of GM-CSF, IL-1β, TNFα, IL-23α and IL-12β to the infection with the wild-type S. Enteritidis when compared with the non-infected PAMs and despite lower invasiveness, PAM infection with the S. Enteritidis ΔSPI1 mutant further significantly increased transcription of these genes (Table 3). This evidence concerns the gene TNF and infection.