Our study investigates a more readily translatable method to interfere with FZD7/Wnt3 interaction, by using the extracellular peptide of FZD7 (named soluble FZD7 or sFZD7) expressed and purified from E. coli to inhibit Wnt/β-catenin-mediated signaling in human HCC cell lines. The gene discussed is FZD7; the disease is hepatocellular carcinoma.