Although, we did not observe the blunting of TF diet-induced weight gain, hyperinsulinemina, and hyperglycemia in mice with loss of function mutation in TLR4, our results in this study do not directly contradict the previously proposed connection between saturated fatty acids and upregulation of TRL4 down-stream signaling leading to insulin resistance and inflammation. The gene discussed is TLR4; the disease is Insulin resistance.