Consequently, considering the critical value of orthotopic human GBM animal models with high invasiveness and EGFR overexpression in preclinical and translational cancer research, in the present work, we establish the intracranial xenograft models by orthotopic retransplantation of human GBM solid tissues maintained as xenografts via serial passaging sc in the flanks of nude mice and report whether the intracranial xenograft models can retain histopathological features and genetic properties of the clinical GBM with high invasiveness and EGFR overexpression. This evidence concerns the gene EGFR and cancer.