Here, our RT-PCR and western-blot analysis demonstrated the presence of EGFR gene overexpression in all GBM xenografts compared with its original tumors, suggesting that the intracranial or flank xenograft models can retain the genetic property of EGFR overexpression in clinical GBM by orthotopic or heterotopic retransplantation of human GBM solid tissues. The gene discussed is EGFR; the disease is glioblastoma.