Highest IL-12 production was observed in mice vaccinated with DNA-prime/Protein-boost group, which was significantly higher in mice vaccinated with either gp63 alone (p<0.001), or in combination with CpG (p<0.05), or rgp63 in association with CpG (p<0.001) in both short and long-term studies before challenge infection (Figure 4A and B). The gene discussed is LMLN; the disease is infection.