In accordance with this hypothesis, presenilin-1 mutant knock-in mice exhibit greater severity of brain injury and poorer behavioural outcome after focal cerebral ischemia/reperfusion [36]; moreover, neuronal elevation of intracellular calcium levels is enhanced after glucose deprivation and chemical hypoxia in cortical cell cultures from the same animals [36]. The gene discussed is PSEN1; the disease is Cerebral ischemia.