Figure 3 shows a comparison of four EmCa tissues (T) with four normal endometria (N) for the five biomarker candidates with β-actin serving as a loading control. Furthermore, immunohistochemical analyses in an independent set of tissue samples (n = 5 each) revealed intense cytoplasmic and/or nuclear immunostaining of S100A6 protein in tumor cells of endometrioid EmCa tissue sections (typical results are shown in Figure 4), whereas no significant immunostaining was observed in the epithelial cells of normal proliferative endometria. Here, S100A6 is linked to neoplasm.