Medulloblastoma-associated CSCs selected by serum-free medium with bFGF and EGF can form 3D spheroids and display enhanced self-renewal and highly co-expressed stem cell genes (Oct4, Nanog, Nestin, and Musashi-1) as well as anti-apoptotic genes (Bcl-2 and Bcl-XL) [76]. The gene discussed is POU5F1; the disease is medulloblastoma.