SOX2 knockdown by Pre-miR-126 and siRNA was confirmed by Western blot analysis in SOX2-expression-positive gastric cancer cell lines MKN45 and HSC43 (Figure 2A), and the subsequent expression changes of the putative SOX2 downstream target genes were preliminarily analyzed by RT-PCR in these cell lines, and then by quantitative real time RT-PCR in HSC43 cells. This evidence concerns the gene SOX2 and gastric cancer.