This study is the first to demonstrate that FECH siRNA, which is a non-invasive and safe molecular agent because it led to an accumulation of intrinsic and non-toxic PpIX within the cytoplasm, offers the promise of being an exciting new tool with great potential to enhance the efficiency of 5-ALA fluorescence and 5-ALA–PDT in glioma cells. This evidence concerns the gene FECH and central nervous system cancer.