Moreover, transient expression of EGFP-Hec1 in other mammalian cell lines, such as osteosarcoma (U2OS) or diploid colon cancer (HCT116) cells generated the same phenotypes, suggesting that mitotic disruption brought about by the presence of the chimeric protein was a general phenomenon (Figure S4). This evidence concerns the gene NDC80 and malignant colon neoplasm.