To establish whether tumor-derived soluble factors are sufficient to induce differential expression of miR-101 and EZH2, HBMVEC cells were cultured in endothelial basal medium (EBM), EBM supplemented with an angiogenic cocktail (EGM), or EBM conditioned culture medium derived from U87 glioblastoma cells (U87cm). The gene discussed is EZH2; the disease is neoplasm.