To validate our findings, we examined whether ALKBH3 gene silencing in vivo affects squamous cell carcinoma using the human lung squamous cell carcinoma cell line RERF-LC-AI, but no significant differences in tumour formation were observed between the two groups injected with either ALKBH3 siRNA or control RNA (data not shown). This evidence concerns the gene ALKBH3 and squamous cell lung carcinoma.