In this report, we investigated the impact of disrupting C2-O-sLeX synthesis on the distribution of sLeX on core 2 O-glycans versus N-glycans on colon and hepatic carcinoma cell lines, their E-selectin binding capacity, and the influence on their invasive properties as initial steps to elucidate the function of these carbohydrates in metastasis. Here, SELE is linked to hepatocellular carcinoma.