GCNT1 and neoplasm: Using a Matrigel invasion assay that mimics active invasion of tumor cells across a basement membrane, we found that after 48 hours, the number of C2GnT1 shRNA-transduced LS174T cells that invaded across the Matrigel membrane was significantly decreased compared to LS174T cells transduced with scrambled shRNA (mean number of cells/field  = 27 versus 162, p<0.0001) (Fig. 6A, C, and D).