The tendency of the Htt fragments to aggregate hampered the efforts to make this assay fully quantitative using Htt protein standards, however we attempted to obtain a relative quantitative sensitivity measurement for mtHtt that is normalized to the signal that estimates the presence of tHtt, thus mt/tHtt signals, using purified 548Qn Htt fragments (Weiss et al [5], [4]) spiked into control BC samples (Fig. 7).The results using these Htt fragments indicate a narrow dynamic range for detecting mt/tHtt: 33.4-16.1 ng/mL for both the 548Q46 and 548Q72 Htt fragments. This evidence concerns the gene HTT and breast cancer.