Consequently, we found that the release of HCV Core or the infectivity of HCV into the culture supernatants was significantly suppressed in the Brox knockdown cells 4 days after HCV-JFH1 infection (Fig. 5E and 5F), while the RNA replication of HCV-JFH1 was marginally affected in the knockdown cells (Fig. 5G) in spite of the very effective knockdown of Brox mRNA (Fig. 5D), suggesting that Brox is also required for the infectious HCV production. The gene discussed is BROX; the disease is infection.