Immunohistochemical studies of all CUCC buttons, including 5 eyes from keratoconus patients, 1 eye with corneal ectasia due to previous LASIK surgery and 3 eyes with corneal scars and irregular astigmatism following RK, underwent immunohistochemical staining with mAb 4A4 against ΔNp63, CX 43, and Krt3 monoclonal antibody (Table 2). Here, KRT3 is linked to Astigmatism.