TTR and Cognitive impairment: In fact, whereas most of the significant transthyretin 2D-DIGE gel features were decreased in AD, the global transthyretin levels measured by ELISA in the ‘discovery’ and ‘validation’ cohorts were actually mildly increased in groups with cognitive impairment (CDR>0) relative to those without (CDR 0) (Figures 4 and 5).