It is known that enhanced caspase 8 recruitment to the DISC is essential for overcoming TRAIL resistance in human hepatocellular carcinoma cells [49], therefore it is possible that TNFα and IFN-γ may enhance caspase 8 association with the DISC or mediate DISC conformation to alter the caspase 8 cleavage kinetics in human colon carcinoma cells, which remains to be determined. Here, TNF is linked to colon carcinoma.