Additionally, Realtime analysis revealed a significant reduction of lung Nogo-B mRNA expression in chronic asthmatic mice (Figure 1G), in accordance with this, Western blotting analysis of the total proteins collected from the lung homogenates showed that Nogo-B expression was approximately 3.08-fold lower in chronic asthmatic mice than in control mice (Figure 1H-I), indicating that Nogo-B may play a role in airway smooth muscle remodeling in asthma. Here, RTN4 is linked to asthma.