To determine the frequency of commonly available cancer cell lines that have the hallmarks of O2-insensitivity, we used a reverse phase protein array (RPPA) and screened 57 different ovarian cancer cell lines for the levels of 14-3-3 σ and CDC2, as well as phospho-RB as an indicator of CDC2 activity. The gene discussed is CDK1; the disease is ovarian carcinoma.