A member of the Gram-negative bacteria-binding protein (GNBP) family (AAEL007064), the Toll-like receptor Toll5A (AAEL007619) and the MYD88 factor (AAEL007768), were also up-regulated in infected salivary glands (Table 1), suggesting that the Toll pathway is activated in Ae. aegypti salivary gland anti-dengue defense mechanisms. This evidence concerns the gene TLR4 and dengue disease.