Here we show for the first time that chronic exposure of A549 cells (NSCLC cells) to TGF-β1 led to the acquisition of EMT phenotype with concomitant up-regulation of sonic hedgehog (Shh) both at the mRNA and at the protein levels, which is consistent with findings in another NSCLC cell line (H2030). The gene discussed is TGFB1; the disease is non-small cell lung carcinoma.