PKC is also activated by diacylglycerol (DAG) [82], and it is possible that factors such as hyperglycemia known to cause abnormal enhancement of the DAG concentration and therefore activating PKC and NAD(P)H oxidase in endothelial cells (as will be discussed later) also could play a role as contributory causes of hyperalgesia mediated by C-fibre PKC. This evidence concerns the gene PRRT2 and Hyperglycemia.