We proceeded to perform luciferase assays to investigate the effect of the expression of wild-type versus various mutant Rasd1 proteins on Ear2-mediated renin promoter activity in three cell lines, including COS-7 (monkey kidney fibroblast cell line), As 4.1 (mouse juxtaglomerular cell line) and Neuro2a (mouse neuroblastoma cell line). Here, REN is linked to neuroblastoma.