The results showed that exposure of glioma cells to either BO-1051 or irradiation (2 Gy) resulted in a significant increase of γ-H2AX foci at 1 h that was sustained for 6 h, and then the γ-H2AX foci declined to almost basal level at least 24 h after irradiation or drug removal (Figure 5A and 5B). This evidence concerns the gene H2AX and glioma.