Evident Patch1, Smo, Gli1, Gli2, and Gli3 expressions were found in PIN lesions (Figure 2F to 2J) and CaP (Figure 2K to 2O) of the pCX-shh-IG-injected prostates, in contrast to the almost absence of expression in the vehicle controls (Figure 2A to 2E), except that few basal cells and stromal cells were Smo+ (Figure 2B). Here, GLI2 is linked to prostate intraepithelial neoplasia.