TRPV1 and neuropathic pain: Based on the over-expressed endocannabinoid/endovanilloid biomarkers and elevated glutamate levels, we decided to perform in SNI rats a repeated daily systemic treatment with AA-5-HT, which we had previously demonstrated to produce anti-allodynic and anti-hyperalgesic effects in the chronic constriction injury (CCI) neuropathic pain model via both indirect activation/desensitization of TRPV1 and activation of cannabinoid CB1 receptors, following elevation of AEA and 2-AG levels, and direct TRPV1 receptor antagonism [49].