Provided that only a single locus has to be analyzed at regular intervals, and that the differentially methylated region of interest is well characterized and quite small, as is the case for routine diagnostic applications (e.g., analyzing MGMT gene methylation in glioblastoma [33] or hMLH1 gene methylation in colorectal carcinoma [34]) conventional pyrosequencing remains the method of choice. This evidence concerns the gene MLH1 and glioblastoma.