Specifically, whereas the basal levels of activity for recombinant preparations of GAC are typically < 5% of the activity measured in the presence of 50-100 mM inorganic phosphate, which serves as an allosteric activator of the enzyme, the basal activity measured in mitochondrial fractions from these breast cancer cells is generally 30-50% of the maximal activity assayed in the presence of inorganic phosphate. Here, GLS is linked to breast cancer.