To further reveal the heterogeneity of BRAF mutations within primary melanoma, we examined 10 tumours (one melanoma on chronic sun-damaged skin, four melanomas on non-chronic sun-damaged skin, two acral melanomas and three mucosal melanomas) by the sensitive Mutector assay detecting the BRAFV600E mutation (TrimGen Corporation), as well as by cloning and sequencing of separated alleles (Table 2). The gene discussed is BRAF; the disease is neoplasm.