PROM1 and central nervous system cancer: As shown in Figure 4, we found that in vitro culture of T98G (A, B) and U87MG (C, D) cells in DMEM as monolayer (glioma) and in NBM (gliospheres), dissociated gliosphere cells in NBM (BTSCs) and purified CD133+ gliosphere cells in NBM (CD133+ BTSCs) resulted in a significant increase in viable cell count (A, C) and proliferation (B, D) as measured by WST-1 and 3H thymidine uptake assays, respectively.