To determine whether TLR expression is also found in the fibroblasts in vivo, immunohistochemical studies were performed to localize the expression of TLR3 in skin from the bleomycin-induced skin fibrosis model (Figure 5A), as well as from the skin biopsies of healthy controls and patients with SSc (Figure 5B). This evidence concerns the gene TLR3 and systemic sclerosis.