We report herein that despite the induction of pathogen-reactive T cells, IFN-γ, TNF-α, NOS2, and the preservation of IFN-γ-induced antimicrobial activity in microglia/macrophages, adaptive immunity still required the autophagic pathway to confer in vivo protection against cerebral and ocular toxoplasmosis. This evidence concerns the gene IFNG and ocular toxoplasmosis.