Indeed, while IFN-γ, TNF-α and NOS2 are key for resistance against chronic toxoplasmosis, we report that the expression of these mediators does not appear sufficient to prevent ocular and cerebral toxoplasmosis since CD40 as well as the autophagy proteins Beclin 1 and Atg7 are required for protection against the disease. Here, CD40 is linked to cerebral toxoplasmosis.