To test whether treatment with pioglitazone was remembered in subcultured myotubes from subjects with PCOS, we compared the metabolic characteristics of myotubes established from PCOS subjects before and after pioglitazone treatment and further assessed by quantitative real-time PCR, the expression of genes which are not directly associated to the lipid and glucose metabolism; solute carrier family 22 (SLC22A16), pleckstrin (PLEK) and tau tubulin kinase 2 (TTBK2). Here, SLC22A16 is linked to polycystic ovary syndrome.