A previous microarray study of in vivo skeletal muscle tissue of the same PCOS subjects before and after pioglitazone treatment [19] found these transcripts significantly differentially expressed; pleckstrin (PLEK) (p = 0.005, FC  = 4.6), solute carrier family 22 (SLC22A16) (p = 0.03, FC  = 4.2), and tau tubulin kinase (TTBK2) (p = 0.00002, FC  = −4.0). This evidence concerns the gene TTBK2 and polycystic ovary syndrome.