In order to test whether the impairments in vivo is only translated into myotubes established from subjects with the highest degree of hyperandrogenism or hyperinsulinemia, the PCOS group was subdivided into two groups, individuals with the fourteen lowest (range 0.77–1.89 nmol/l) and fourteen highest (range 1.91–4.24 nmol/l) total testosterone levels respectively, or fourteen lowest (range 38.0–73.0 pmol/l) and fourteen highest (range 79.7–236.3 pmol/l) insulin levels respectively and further tested for differences in metabolic phenotype. This evidence concerns the gene INS and hyperinsulinism.