AHCYL2 and hepatocellular carcinoma: The genes selected for additional analysis using bisulfite sequencing PCR (BSP) were coronin, actin binding protein 2ba (coro2ba) (hypermethylated in HCC), insulin-like growth factor binding protein 1b (igfbp1b) and angiopoietin-like 3 (angptl3) (hypomethylated in HCC), no-tail (ntla) (no change) and S-adenosylhomocysteine hydrolase-like 2 (ahcyl2) and inhibitor of DNA binding 2 (id2a) (showed no statistically significant change).