BBC3 and infection: Additionally, we evaluated lung histology during intratracheal infection with an alternate bacterial pathogen, Staphylococcus aureus, and determined that the infected Puma−/− mice were again more prone to abscess development than the Puma+/+ infected mice (4.5 lesions/mouse vs. 2 lesions/mouse) (Figure 2, C and D vs. G and H).