TNF and asthma: 3.4. Frequencies of CD4+ and CD8+ T Cell producing IL-2, IL-4, IL-10, IL-13, IFN-γ, and TNF-α before and after In Vitro Stimulation with Phorbol 12-Myristate 13-Acetate and Ionomycin in Patients with Different Degree of Severity of Asthma