Apart from demonstrating the function of c-Myb in the regulating OPN expression in HCC cells, we also showed that down-regulation of c-Myb by siRNA decreased OPN expression and also inhibited the migration and invasion of HCCLM6 cell in vitro, indicating that modulating OPN expression by targeting c-Myb might be a new approach for intervening HCC invasion and metastasis. This evidence concerns the gene SPP1 and hepatocellular carcinoma.