MAP1LC3A and infection: Additionally, RAW 264.7 macrophages transfected with green fluorescent protein (GFP) fused to the autophagosome protein LC3 (GFP-LC3) [20] showed a significant increase in GFP-LC3 puncta formation when infected with Mtb-Δeis at a multiplicity of infection (MOI) of 10 (over levels in cells infected with Mtb-WT or Mtb-c-eis at the same bacterial load; Fig. S1A).