As anticipated, pretreatment with the ROS scavengers [N-acetyl cysteine (NAC), diphenyleneiodonium (DPI), catalase and tiron (4,5-dihydroxy-1,3-benzene disulfonic acid-disodium salt); for 1 h before infection] prevented Mtb-Δeis-induced autophagosome accumulation in BMDMs (Fig. 3A) and RAW 264.7 cells transfected with GFP-LC3 (Fig. S4A). This evidence concerns the gene CAT and infection.