FLII and neoplasm: To assess how the host inflammatory response changes as tumor expansion proceeds, we crossed V12RAS+/Fli:eGFP+ fish with Tg(LysC:DsRed) and chose larvae at 4–5 dpf that had already developed more than 15 V12RAS+ clones within the skin of one side of the flank, but with no more than 15 cells in any one individual clone.