Transmigration of Melanoma-7 cells (Cell Tracker-568) through monolayers with 15 or 25% CXCL9-transfected, 18% mock-transfected or non-transfected HUVECs (transfection efficiency defined by FACS analysis (EGFP-positive cells)) was allowed for 8 h. Here, CXCL9 is linked to melanoma.