ESR1 and breast cancer: Another study in patients of breast cancer with secondaries in liver found that factors that significantly predicted a poor prognosis on univariate analysis included symptomatic liver disease, deranged liver function tests (LFTs), the presence of ascites, histological grade 3 disease at primary presentation, advanced age, estrogen receptor (ER) negative tumors, CEA of over 1000 ng/ml and multiple vs single liver metastases.