These significant findings persisted after adjustment of age for the association between obesity and methylation status of the CpG site in the UBASH3A gene (P = 0.008) and TRIM3 gene (P = 0.001), but changed to borderline significance for HIPK3 gene (P = 0.05), and disappeared for the CpG site in the CDH5 gene (P = 0.095). This evidence concerns the gene TRIM3 and obesity due to melanocortin 4 receptor deficiency.