Our qPCR analysis included GIST with wild type sequences in the hot-spot regions of KIT and PDGFRA (wt-GIST), KIT exon 9 and exon 11 mutated samples, PDGFRA exon 18 mutated samples, NF-1-associated wt-GIST and normal tissue controls. The gene discussed is KIT; the disease is gastrointestinal stromal tumor.