After multivariable adjustment for sex, BMI, systolic blood pressure, alcohol use, waist-hip ratio, diabetes, fasting glucose, total cholesterol to HDL radio, triglycerides, IL-6, adiponectin, and ghrelin, the trend of decreasing least squares means of ALT with age remained consistent and statistically significant (p<0.0001). This evidence concerns the gene GHRL and diabetes mellitus.