Figure 6A shows the relative level of MMP1 expression in cells recovered from the control group and the silenced group. The relative level of MMP1 expression in control and silenced isolated cells (97% down) was similar to the original extent of MMP1 silencing prior to implantation indicating stability of the shRNA effect through the in vivo tumor growth and metastasis. Furthermore, peri-tumoral MMP1a & MMP1b production by murine stromal tissue was assessed with laser capture microdissection and QPCR, figure 6B. This evidence concerns the gene MMP1 and neoplasm.