However, with the exception of Prn and BrkA, most of other known protective antigenssuch as PT, FHA, Fimbriae 2 and 3, and main pertussis virulence factors includingTCT, LPS, CyaA and DNT always seem to escape detection by 1-D or 2-D immunoblotting[20], [28]. This evidence concerns the gene F2 and pertussis.