In conclusion, the results of the present study show: 1) stimulation of TLR2 and TLR4 induces greater expression of IL-6, IL-8 and ICAM-1 in T1D CAECs, 2) the enhanced inflammatory responses to TLR2 and TLR4 agonists in diabetic CAECs correlate with augmented NF-κB activation in the absence of an alteration of cellular TLR2 and TLR4 protein levels, and 3) insulin alone is insufficient to suppress the hyper-inflammatory responses to both TLR2 and TLR4 agonists in diabetic CAECs. Here, TLR4 is linked to type 1 diabetes mellitus.